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Expression validation of <t>LIFR</t> in CRSwNP. ( A ) Transcriptional levels of biomarkers (LIFR, LIF, IL-5, IL-13) in PR versus non-PR groups. ELISA quantification of LIFR concentrations in both ( B ) serum and ( C ) NF of PR patients compared to non-PR and control groups. ( D ) Representative images of H&E and LIFR immunohistochemical staining (Magnification ×400). ( E ) Quantification of LIFR protein expression intensity (n=6/group). ( F and G ) scRNA-seq analysis of CRSwNP tissues showing <t>LIFR</t> <t>expression</t> patterns across cell populations (left: cell type clusters; right: LIFR expression). ( H ) Immunofluorescence of LIFR (red) co-localization with vascular endothelial cells (CD31+, green) and nuclear staining (DAPI, blue) at ×400 magnifications (n=6/group). * P < 0.05, ** P < 0.01, **** P < 0.0001.
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Expression validation of <t>LIFR</t> in CRSwNP. ( A ) Transcriptional levels of biomarkers (LIFR, LIF, IL-5, IL-13) in PR versus non-PR groups. ELISA quantification of LIFR concentrations in both ( B ) serum and ( C ) NF of PR patients compared to non-PR and control groups. ( D ) Representative images of H&E and LIFR immunohistochemical staining (Magnification ×400). ( E ) Quantification of LIFR protein expression intensity (n=6/group). ( F and G ) scRNA-seq analysis of CRSwNP tissues showing <t>LIFR</t> <t>expression</t> patterns across cell populations (left: cell type clusters; right: LIFR expression). ( H ) Immunofluorescence of LIFR (red) co-localization with vascular endothelial cells (CD31+, green) and nuclear staining (DAPI, blue) at ×400 magnifications (n=6/group). * P < 0.05, ** P < 0.01, **** P < 0.0001.
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Expression validation of <t>LIFR</t> in CRSwNP. ( A ) Transcriptional levels of biomarkers (LIFR, LIF, IL-5, IL-13) in PR versus non-PR groups. ELISA quantification of LIFR concentrations in both ( B ) serum and ( C ) NF of PR patients compared to non-PR and control groups. ( D ) Representative images of H&E and LIFR immunohistochemical staining (Magnification ×400). ( E ) Quantification of LIFR protein expression intensity (n=6/group). ( F and G ) scRNA-seq analysis of CRSwNP tissues showing <t>LIFR</t> <t>expression</t> patterns across cell populations (left: cell type clusters; right: LIFR expression). ( H ) Immunofluorescence of LIFR (red) co-localization with vascular endothelial cells (CD31+, green) and nuclear staining (DAPI, blue) at ×400 magnifications (n=6/group). * P < 0.05, ** P < 0.01, **** P < 0.0001.
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Expression validation of <t>LIFR</t> in CRSwNP. ( A ) Transcriptional levels of biomarkers (LIFR, LIF, IL-5, IL-13) in PR versus non-PR groups. ELISA quantification of LIFR concentrations in both ( B ) serum and ( C ) NF of PR patients compared to non-PR and control groups. ( D ) Representative images of H&E and LIFR immunohistochemical staining (Magnification ×400). ( E ) Quantification of LIFR protein expression intensity (n=6/group). ( F and G ) scRNA-seq analysis of CRSwNP tissues showing <t>LIFR</t> <t>expression</t> patterns across cell populations (left: cell type clusters; right: LIFR expression). ( H ) Immunofluorescence of LIFR (red) co-localization with vascular endothelial cells (CD31+, green) and nuclear staining (DAPI, blue) at ×400 magnifications (n=6/group). * P < 0.05, ** P < 0.01, **** P < 0.0001.
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Expression validation of LIFR in CRSwNP. ( A ) Transcriptional levels of biomarkers (LIFR, LIF, IL-5, IL-13) in PR versus non-PR groups. ELISA quantification of LIFR concentrations in both ( B ) serum and ( C ) NF of PR patients compared to non-PR and control groups. ( D ) Representative images of H&E and LIFR immunohistochemical staining (Magnification ×400). ( E ) Quantification of LIFR protein expression intensity (n=6/group). ( F and G ) scRNA-seq analysis of CRSwNP tissues showing LIFR expression patterns across cell populations (left: cell type clusters; right: LIFR expression). ( H ) Immunofluorescence of LIFR (red) co-localization with vascular endothelial cells (CD31+, green) and nuclear staining (DAPI, blue) at ×400 magnifications (n=6/group). * P < 0.05, ** P < 0.01, **** P < 0.0001.

Journal: Journal of Inflammation Research

Article Title: Association of Increased Nasal Fluids-Serum Concordance of Protein Profile with Prognosis in Nasal Polyps

doi: 10.2147/JIR.S567454

Figure Lengend Snippet: Expression validation of LIFR in CRSwNP. ( A ) Transcriptional levels of biomarkers (LIFR, LIF, IL-5, IL-13) in PR versus non-PR groups. ELISA quantification of LIFR concentrations in both ( B ) serum and ( C ) NF of PR patients compared to non-PR and control groups. ( D ) Representative images of H&E and LIFR immunohistochemical staining (Magnification ×400). ( E ) Quantification of LIFR protein expression intensity (n=6/group). ( F and G ) scRNA-seq analysis of CRSwNP tissues showing LIFR expression patterns across cell populations (left: cell type clusters; right: LIFR expression). ( H ) Immunofluorescence of LIFR (red) co-localization with vascular endothelial cells (CD31+, green) and nuclear staining (DAPI, blue) at ×400 magnifications (n=6/group). * P < 0.05, ** P < 0.01, **** P < 0.0001.

Article Snippet: After 1 h blocking with 10% BSA at room temperature, sections were incubated overnight at 4°C with primary antibodies, including neutrophil elastase (NE; Abcam #ab68672) for neutrophil quantification or LIFR (Origene #TA386958) for LIFR expression detection, followed by DAB substrate.

Techniques: Expressing, Biomarker Discovery, Enzyme-linked Immunosorbent Assay, Control, Immunohistochemical staining, Staining, Immunofluorescence